… ) In vivo UV laser footprinting of the Pseudomonas putida sigma 54 Pu promoter reveals
that integration host factor couples transcriptional activity to growth phase. …

… -phase sigma factor RpoS and integration host factor (IHF). … I footprint assay we have identified
a binding site of P. putida … mobility shift and DNase I footprint studies revealed that Fis out-…

… putida have been examined in a variety of growth stages and culture … The results from this
experiment revealed that neither the … caused by the integration host factor (3, 7, 31). Sigma …

… This is revealed by the fact that overproducing σ 54 largely … abolish growth phase-dependent
control of transcription, … while exhibiting somewhat lower transcriptional activity on the whole…

… Integration of the plasmid into the chromosome was selected … simple assay system, as it
reveals the action of glucose while … 11) and the growth phase control of other σ 54 promoters (38)…

… Crc shows similarity to a family of nucleases that have highly conserved residues at their …
growth media. At mid-exponential phase (turbidity of 0.5 to 0.6), transcription from promoter …

… genes, a transcriptional fusion containing the PbenA promoter … exponential phase of growth
and increased when the growth … plot shows the values obtained as a function of cell growth (…

… Footprinting assays revealed that there are two IHF binding … To test a possible effect of
transcription from this promoter on the … cycle (for instance, IHF levels are increased at stationary …

… generated by growing cells to late logarithmic growth phase in … rather facilitate transcription
from the mutS promoter by a so … The results of the current study revealed that the transcription …

… In vivo UV laser footprinting of the Pseudomonas putidasigma 54Pu promoter reveals
that integration host factor couples transcriptional activity to growth phase. J Biol Chem 2002;277:…